Comparison between phenotype and molecular resistance characteristic in Staphylococcus epidermidis isolates from wound infections in Al-Basrah province, Iraq
DOI:
https://doi.org/10.21533/pen.v9.i2.793Abstract
Background:Staphylococcus epidermidis is considered the upper respiratory tract's human skin flora and mucosal membrane and displays low pathogenic capacity in healthy individuals. Drug-resistant strains can be identified as a natural result of the microflora through antibiotic therapy and are a possible cause of pathogenic strain resistance genes.
Methods:Culture, biochemical analysis and Vitek®2 Utilizing for identified the One hundred and fifty swab sample was collected from different wounds infected. S. epidermidis strain's ability to resist antibiotics was tested using a disk diffusion method. Result of antibiotic sensitivity test was confirmed and supported by Vitek®2 system. Also, the PCR antibiotic resistance gene was detected.
Results: Out of 150 swab samples, twelve were positive for S. epidermidis.. Disc method was shown the 75%,66.7%,83.3% and 58.3% harboured highest prevalence of antimicrobial resistance against penicillin, oxacillin, cefoxitin and erythromycin respectively.While the moderate prevalence 50.7%,41.7% and 33.3% of resistance against tetracycline,clandomycin and ciprofloxacin respectively. Furthermore, lowest incidence was shown the 25% for both of resistance against rifampin, and gentamycin. The Vitek®2 system was confirmed and support antibiotic sensitivity test. A most frequently found antibiotic resistance genes amongst S. epidermidis strains, according to the findings, were mecA (91.7%), blaZ (91.7) , ermA (16.7%),ermB (25%),ermC (25%), tetM (25%), tetK (33.3 %) and aacA-aphD (41.7%) respectively. All S. epidermidis strain doesn't have ,vanA and vanB antibiotic resistance gene.
Conclusion:Frequency of resistance to antibiotic should be detected more than one method , and used the Vitek®2 system detected the antibiotic resistance gave better support for result. Additional PCR technique, actually very important to detect antibiotic resistance genes of S. epidermidis strains.
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