Association of interleukin-6 level and DNA polymorphism in type 2 diabetic patients in Kirkuk city

Abstract

The basic inflammatory factor interleukin-6 plays an important role in the inflammatory response. It has been shown that IL-6 has a role in insulin resistance and T2DM. The IL-6 gene is found on chromosome 7p15-p21 in humans, it works via a receptor composed of two components, the IL-6 receptor, and gp130. Single nucleotide polymorphisms in the regulatory areas of IL-6R genes influence their expression levels and are linked to a higher risk of T2DM. There were 80 T2DM patients and 80 healthy people enrolled in the present work. Fasting blood glucose levels, HbA1C, and IL-6 concentrations were estimated in both groups. The allelic frequencies of 2 single nucleotide polymorphisms (rs2229238, rs4845625) were identified by PCR-SSP. In this study, we found that the fasting blood sugar levels, HbA1c, and IL-6 levels were statistically highly significant in patients in comparison with controls. In regarding the association of genotypic (CC, CT, TT) and allelic frequencies of rs2229238, and rs4845625 polymorphism of the gene IL6R, and prevalence of diabetes mellitus. The results of PCR-SPP products of rs2229238 (C/T) of the IL6R gene indicated the significant correlation the rs4845625 IL6R polymorphism with T2DM, where no significant association was observed between these genotypes (CC, CT, TT) and allelic (T) frequencies of rs2229238 as a risk factor and T2D.