Genotyping of human papillomavirus high-risk types and correlation with potential risk factors

Abstract

Human papillomavirus (HPV) is one of the most common sexually transmitted disease (STD) worldwide. HPV is a
small, double-stranded DNA virus that infects mucosal and coetaneous epithelia trough tiny cuts and abrasion,
exposed by the cells of the basal layer. If diagnosed on time HPV can be successfully treated, however, in some cases
it can lead to the development of tumor. Most of cervical tumors contain HPV DNA, and majority of them contain
high-risk types HPV16 and HPV18. Different risk factors are associated with HPV infection, including behavioral
and biological predispositions. Aim of this study is to genotype potentially infected patients on high-risk types HPV
DNA and to correlate the results with patient’s different biological and lifestyle factors. For this purpose 20
gynecological smear samples were collected from women, previously subjected to the survey. Methodology included
DNA extraction and real-time polymerase chain reaction (RT-PCR). Results showed that out of 20 patients five were
positive for high risk HPV. Four of five positive patients were positive on HPV16 type of which one had HPV16
together with others high risk types. One of five positive patients was positive on HPV18 type and other high risk
types not identified. Final outcome indicates the correlation of potentially endangered patients with specific sexual
behavior and lifestyles, and furthermore represent the general consensus and awareness level this disease has on the
public.